Adenovirus: Methods and Protocols by Miguel Chill?n (English) Hardcover Book

Description: Adenovirus by Miguel Chillón, Assumpció Bosch Rev. ed. of: Adenovirus methods and protocols / edited by William S.M. Wold, Ann E. Tollefson. 2nd ed. c2007. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool. Back Cover Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool. Table of Contents Biophysical Methods to Monitor Structural Aspects of the Adenovirus Infectious Cycle.- Proteome Analysis of Adenovirus Using Mass Spectrometry.- Capsid Modification Strategies for Detargeting Adenoviral Vectors.- Use of Dodecahedron "VLPs" as an Alternative to the Whole Adenovirus.- Study of Adenovirus and CAR Axonal Transport in Primary Neurons.- The Use of Chromatin Immunoprecipitation (ChIP) to Study the Binding of Viral Proteins to the Adenovirus Genome In Vivo.- DNA Microarray to Analyze Adenovirus-Host Interactions.- Determination of the Transforming Activities of Adenovirus Oncogenes.- Oncolytic Adenovirus Characterization: Activity and Immune Responses.- The Analysis of Innate Immune Response to Adenovirus Using Antibody Arrays.- Engineering Adenovirus Genome by Bacterial Artificial Chromosome (BAC) Technology.- Construction, Production, and Purification of Recombinant Adenovirus Vectors.- Scalable Production of Adenovirus Vectors.- Canine Adenovirus Downstream Processing Protocol.- Production of High-Capacity Adenovirus Vectors.- Production of Chimeric Adenovirus. Feature Includes cutting-edge methods and protocols for adenovirus research Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Details ISBN1627036784 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2013 Edition 3rd ISBN-10 1627036784 ISBN-13 9781627036788 Format Hardcover Publication Date 2013-10-17 Imprint Humana Press Inc. Place of Publication Totowa, NJ Country of Publication United States DEWEY 579.2443 Short Title ADENOVIRUS 2014/E 3/E Language English Media Book Series Number 1089 Pages 247 Subtitle Methods and Protocols DOI 10.1007/978-1-62703-679-5 AU Release Date 2013-10-17 NZ Release Date 2013-10-17 US Release Date 2013-10-17 UK Release Date 2013-10-17 Author Assumpció Bosch Edition Description 3rd ed. 2014 Alternative 9781493963256 Edited by Assumpció Bosch Audience Professional & Vocational Illustrations 15 Illustrations, color; 22 Illustrations, black and white; XI, 247 p. 37 illus., 15 illus. in color. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96369500;

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