Post-translational Modifications of Proteins: Tools for Functional Proteomics by

Description: Post-translational Modifications of Proteins by Christoph Kannicht This is a fully up-dated and expanded practical guide to protein structure-function relationships. The book is the most comprehensive volume on PTM analysis available. This important area of research is brought up-to-date by the leading scientists in the field. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This is a fully up-dated and expanded practical guide to protein structure-function relationships. This important area of research is brought up-to-date by the leading scientists in the field. The compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology(TM) series. Comprehensive and cutting edge, the book serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries. Notes This is a fully up-dated and expanded practical guide to protein structure-function relationships and it represents the most comprehensive volume on PTM analysis available. This important area of research is brought up-to-date by the leading scientists in the field. The compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology(TM) series. Comprehensive and cutting edge, the book serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries. Back Cover The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition, this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology(tm) series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries. Table of Contents Disulfide Bond Mapping by Cyanylation-induced Cleavage and Mass Spectrometry.- Detection of Post-translational Modifications by Fluorescent Staining of Two-Dimensional Gels.- Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods.- Analysis of Tyrosine-O-Sulfation.- ?-Amidated Peptides: Approaches for Analysis.- ?-Glutamate and ?–Hydroxyaspartate in Proteins.- Lysine Hydroxylation and Cross-linking of Collagen.- Mass Spectrometric Determination of Protein Ubiquitination.- Analysis of Sumoylation.- Detection and Analysis of Protein ISGylation.- Analysis of Methylation, Acetylation, and other Modifications in Bacterial Ribosomal Proteins.- Analysis of S-Acylation of Proteins.- Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa.- 2-Dimensional Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern.- Carbohydrate Composition Analysis of Glycoproteins by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag.- Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB).- Separation of N-Glycans by HPLC.- Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry – detection of Lewis X Structures.- Immunochemical Methods for the Rapid Screening of the O-Glycosidically Linked N-Acetylglucosamine Modification of Proteins.- Analysis of O-Glycosylation.- Characterization of Site-specific N-Glycosylation.- Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag.- Comparability and Monitoring Immunogenic N-linked Oligosaccharides from Recombinant Monoclonal Antibodies from Two Different Cell Lines using HPLC with FluorescenceDetection and Mass Spectrometry.- Mass Spectrometry and HPLC with Fluorescent Detection-Based Orthogonal Approaches to Characterize N-Linked Oligosaccharides of Recombinant Monoclonal Antibodies.- Web-based Computational Tools for the Prediction and Analysis of Post-translational Modifications of Proteins. Long Description Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition, is a compilation of detailed protocols needed to detect and a- lyze the most important co- and post-translational modifications of proteins. For reasons of simplicity, although not explicitly mentioned in the title, both kinds of modifications are covered, regardless of whether they occur during or after b- synthesis of the protein. My intention was to cover the most significant protein modifications, focusing on the fields of protein function, proteome research, and characterization of pharmaceutical proteins. The majority of all proteins undergo co- and/or post-translational modifications. The proteins polypeptide chain may be altered by proteolytic cleavage, formation of disulfide bonds, or covalent attachment of phosphate, sulfate, alkyl groups, l- ids, carbohydrates, polypeptides, and others. Knowledge of these modifications is extremely important, because they may alter physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the fu- tion of the proteins. Moreover, the modification itself can act as an added functional group. Examples of the biological effects of protein modifications include: ph- phorylation for signal transduction, ubiquitination for proteolysis, attachment of fatty acids for membrane anchoring or association, glycosylation for protein ha- life, targeting, cell-cell and cell-matrix interaction, and carboxylation in protein- ligand binding, to name just a few. Full understanding of a specific protein structure-function relationship requires detailed information not only about its amino acid sequence, which is determined by the corresponding DNA sequence, but also on the presence and structure of protein modifications. Feature A fully up-dated and expanded practical guide to protein structure-function relationships Most comprehensive volume on PTM analysis available today Contains cutting-edge methods in the most high interest areas of the field Provides easy-to-follow, readily reproducible protocols and time-saving notes Description for Sales People This is a fully up-dated and expanded practical guide to protein structure-function relationships and it represents the most comprehensive volume on PTM analysis available. This important area of research is brought up-to-date by the leading scientists in the field. The compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology(tm) series. Comprehensive and cutting edge, the book serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries. Details ISBN1588297195 Short Title POST-TRANSLATIONAL MODIFICATIO Pages 390 Language English Edition 2nd ISBN-10 1588297195 ISBN-13 9781588297198 Media Book Format Hardcover Series Number 446 Year 2008 Subtitle Tools for Functional Proteomics Place of Publication Totowa, NJ Country of Publication United States Edited by Christoph Kannicht Imprint Humana Press Inc. DOI 10.1604/9781588297198;10.1007/978-1-60327-084-7 AU Release Date 2008-04-01 NZ Release Date 2008-04-01 US Release Date 2008-04-01 UK Release Date 2008-04-01 Author Christoph Kannicht Publisher Humana Press Inc. Edition Description 2nd ed. 2008 Series Methods in Molecular Biology Publication Date 2008-04-01 Alternative 9781617377396 DEWEY 572.633 Audience Professional & Vocational Illustrations 128 Illustrations, black and white; XVIII, 390 p. 128 illus. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96288244;

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