RNA Interference Techniques by Scott Q. Harper (English) Paperback Book

Description: RNA Interference Techniques by Scott Q. Harper Non-coding, inhibitory microRNAs have emerged as important modulators of cellular gene expression, through a process called RNA interference (RNAi). FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description Non-coding, inhibitory microRNAs have emerged as important modulators of cellular gene expression, through a process called RNA interference (RNAi). To date, hundreds of conserved and species-specific microRNAs have been identified in organisms ranging from single-celled algae to humans. Many of these tiny RNAs are now known to play fundamental roles in developmental biology and disease pathogenesis. In addition, RNAi has emerged as a technology useful for manipulating gene expression. In RNA Interference Techniques, expert researchers present detailed methods for designing and delivering artificial inhibitory RNAs to neural tissue and for detecting or cloning endogenous microRNAs, all in order to aid investigators attempts to ask basic biological questions or develop therapeutics for dominant neurogenetic disorders, cancer, or viral infection. As a volume in the successful Neuromethods series, the chapters provide authoritative accounts of the most commonly used approaches in the field today. Cutting-edge and concise, RNA Interference Techniques promises to support the vital research in the field of RNAi and miRNAs, ever-continuing to grow rapidly and gain increasing importance in basic and translational biology. Back Cover Non-coding, inhibitory microRNAs have emerged as important modulators of cellular gene expression, through a process called RNA interference (RNAi). To date, hundreds of conserved and species-specific microRNAs have been identified in organisms ranging from single-celled algae to humans. Many of these tiny RNAs are now known to play fundamental roles in developmental biology and disease pathogenesis. In addition, RNAi has emerged as a technology useful for manipulating gene expression. In RNA Interference Techniques , expert researchers present detailed methods for designing and delivering artificial inhibitory RNAs to neural tissue and for detecting or cloning endogenous microRNAs, all in order to aid investigators attempts to ask basic biological questions or develop therapeutics for dominant neurogenetic disorders, cancer, or viral infection. As a volume in the successful Neuromethods series, the chapters provide authoritative accounts of the most commonly used approaches in the field today. Cutting-edge and concise, RNA Interference Techniques promises to support the vital research in the field of RNAi and miRNAs, ever-continuing to grow rapidly and gain increasing importance in basic and translational biology. Table of Contents Designing Hairpin-Based RNAi Shuttles.- Rapid Cloning and Validation of MicroRNA Shuttle Vectors: A Practical Guide.- The Use of Small Non-Coding RNAs to Silence Transcription in Human Cells.- Delivery Strategies for RNAi to the Nervous System.- Cloning Small RNAs.- Profiling the miRNome: Detecting Global miRNA Expression Levels with DNA Microarrays.- High Throughput Profiling of Mature MicroRNA by Real-Time PCR.- In Situ Detection of MicroRNAs in Paraffin Embedded, Formalin Fixed Tissues: Different Methodologies and Co-Localization with Possible Targets.- Tracing of siRNAs Inside Cells by FRET Imaging.- Application of RIP-Chip for the Identification of miRNA Targets. Feature Spotlights a rapidly growing field with increasing importance in basic and translational biology Contains key protocols and clear cut guidance Supports the development of therapeutics for dominant genetic disorders, cancer, or viral infection Details ISBN1493957627 Pages 173 Publisher Humana Press Inc. Series Neuromethods Year 2016 ISBN-10 1493957627 ISBN-13 9781493957620 Format Paperback Publication Date 2016-08-23 Imprint Humana Press Inc. Place of Publication Totowa, NJ Country of Publication United States Edited by Scott Q. Harper DEWEY 572.88 Language English Series Number 58 UK Release Date 2016-08-23 AU Release Date 2016-08-23 NZ Release Date 2016-08-23 US Release Date 2016-08-23 Author Scott Q. Harper Birth 1965 Affiliation Temple University, Philadelphia Position EDITOR Qualifications MD Edition Description Softcover reprint of the original 1st ed. 2011 Alternative 9781617791130 Audience Professional & Vocational Illustrations XII, 173 p. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:126662809;

Price: 253.56 AUD

Location: Melbourne

End Time: 2025-01-07T01:26:04.000Z

Shipping Cost: 9.58 AUD